700+ Growth Factors | 100+ Billion Exosomes

WHAT ARE EXOSOMES?

In Simple Terms :

Exosomes are like tiny packages that cells use to communicate with each other and facilitate the repair and regeneration of tissues. Think of them as the body's natural messengers, delivering important signals to cells to keep them functioning properly and promote healing. Exosomes contain all the growth factors, peptides, proteins and RNA that our body needs to heal itself. Over time, our body's exosomes lose their potency, which can lead to various signs of ageing and health issues such as wrinkles, arthritis, hair loss, grey hair, sore joints and muscles, and the development of serious neurological and immunodeficiency conditions.

Understanding the Science Behind UC-MSC Exosomes

Exosomes are small extracellular vesicles that play a crucial role in cellular communication and the regeneration of tissues.
These natural messengers are pivotal in maintaining cellular function and promoting healing. However, not all exosomes are created equal. At Advanced-Biologics, we specialize in the most advanced form of these powerful entities—UC-MSC (Umbilical Cord Mesenchymal Stem Cell) exosomes, which are derived from the Wharton's Jelly of the umbilical cord.

The difference between stem cells and exosomes lies in their applications and safety profiles. While stem cells, including embryonic and mesenchymal stem cells, have the potential to differentiate into various cell types, they also carry risks such as uncontrolled cell proliferation. In contrast, UC-MSC exosomes, being acellular and not containing any genetic material, do not pose these risks, making them a safer alternative for clinical applications.

Our UC-MSC exosomes are characterized by their high stability, low immunogenicity, and the absence of DNA, which eliminates the risk of tumor formation—a concern associated with stem cell therapies. They are capable of crossing biological barriers efficiently, which enhances their therapeutic potential.


Each exosome batch from Advanced-Biologics contains over 700 active growth factors, ensuring high efficacy with fewer treatments compared to other products on the market. By choosing our UC-MSC exosomes, practitioners are not only opting for a product with unmatched purity and potency but also a product that is rigorously tested and compliant with the highest regulatory standards. This commitment to quality and safety makes Advanced-Biologics a leader in the field of regenerative medicine, offering products that truly enhance patient outcomes.

BIOGENESIS OF EXOSOMES

Exosome biogenesis is a complex process that begins with the formation of early endosomes from the inward budding of the plasma membrane. These early endosomes mature into late endosomes, which then develop into multi-vesicular bodies (MVBs) through the inward budding of their membrane. This process creates intraluminal vesicles (ILVs) within the MVBs.

The endosomal sorting complex required for transport (ESCRT) machinery, comprising around 30 proteins assembled into four complexes (ESCRT-0, -I, -II, and -III), plays a crucial role in regulating the formation of these ILVs. Additionally, proteins like TSG101 and ALIX are involved in exosome biogenesis. Exosomes can also be formed through an ESCRT-independent mechanism.

Once formed, the MVBs can either fuse with lysosomes for degradation or fuse with the plasma membrane, leading to the release of the ILVs as exosomes into the extracellular environment. These exosomes, ranging from 30 to 180 nm in size, contain a diverse array of biomolecules, including proteins, lipids, and RNA, reflecting the composition of their parent cells.

Umbilical cord-derived mesenchymal stem cell (UC-MSC) exosomes possess unique properties that make them particularly attractive for therapeutic applications. These exosomes exhibit high stability, low immunogenicity, and ease of storage and transportation. Additionally, UC-MSC exosomes do not contain DNA or other genetic material, eliminating the risk of uncontrolled cell proliferation or tumour formation associated with stem cell therapies. Their small size allows them to cross biological barriers more efficiently, enhancing their therapeutic potential.

EXOSOME DEPLETION WITH AGE and MSC EXOSOME THERAPY:

As we age, the quality and quantity of our endogenous exosomes decline, contributing to the gradual deterioration of cellular function and tissue regenerative capacity. Hence the onset of wrinkles, arthritis, hair loss, neurodegeneration, cancers and other diseases and disorders.

Exosomes secreted by mesenchymal stem cells (MSCs), particularly those derived from the Wharton's jelly of the human umbilical cord (UC-MSC Exosomes), have emerged as a promising regenerative therapy to counteract this age-related exosome depletion. MSC exosomes are rich in bioactive molecules, including growth factors, cytokines, and regulatory RNAs, that stimulate tissue repair and rejuvenation.

THE REGENERATIVE POWER of EXOSOMES: From Embryonic Development to Therapeutic Applications

Human embryos develop from embryonic stem cells into a fully formed body through a complex process of differentiation and organogenesis, which is heavily influenced by extracellular vesicles called exosomes. Embryonic stem cells are derived from the inner cell mass of the blastocyst, a pre-implantation stage of the embryo. These stem cells are pluripotent, meaning they can give rise to cells of all three germ layers - ectoderm, mesoderm, and endoderm.

As the embryo develops, the pluripotent stem cells undergo a process called gastrulation, where they differentiate into the specialized cell types that will form the various tissues and organs. Signalling molecules like retinoic acid, Wnt, and FGF, as well as transcription factors like the Hox genes, guide this patterning and segmentation process. Importantly, exosomes secreted by the embryonic stem cells and differentiating cells are crucial in mediating these signalling pathways and coordinating complex morphogenesis.

This remarkable self-organization, driven in part by exosomes, allows a single fertilized egg to develop into the intricate, multi-organ human body. However, as we age, the quality and quantity of our endogenous exosomes decline, contributing to the gradual deterioration of cellular function and tissue regenerative capacity.

HARNESSING THE REGENERATIVE POWER of MSC EXOSOMES

Unlike stem cell therapies, MSC exosomes do not contain any genetic material, eliminating the risk of uncontrolled cell proliferation or tumour formation. By introducing these potent, DNA-free exosomes into the body, individuals can experience the regenerative benefits of stem cell therapy without safety concerns.Numerous studies have demonstrated the ability of MSC exosomes to promote the regeneration of various cell types, making this therapy a versatile approach for addressing a wide range of age-related conditions, from neurodegenerative diseases to cardiovascular decline and metabolic disorders.

HUMAN EXOSOME SOURCES

Exosomes can be derived from various sources, including:

  1. Mesenchymal stem cells (MSCs):

Bone marrow-derived MSCs (BM-MSCs8

Adipose tissue-derived MSCs (AT-MSCs)

Umbilical cord-derived MSCs (UC-MSCs)

The majority of the information focuses on exosomes derived from MSCs of different tissue origins, particularly bone marrow, adipose tissue, and umbilical cord. These MSC-derived exosomes have gained significant attention due to their potential therapeutic applications in regenerative medicine and cell-free therapies.

Several studies highlight the isolation, characterization, and proteomic analysis of exosomes from BM-MSCs, AT-MSCs, and UC-MSCs, exploring their similarities, differences, and potential therapeutic implications.

While the search results do not provide an exhaustive list of all possible exosome sources, they emphasize the importance of MSC-derived exosomes, especially those from bone marrow, adipose tissue, and umbilical cord, as promising candidates for regenerative therapies and cell-free therapeutic modalities.

The Difference in Exosome Products:

At Advanced-Biologics, we're proud to offer exosomes that are uncompromised and in full form and function, making them the most effective, pure, potent, consistent, safe and ambitious exosomes available. While other products in the market may rely on conditioned media, plant exosomes, or lyophilized exosomes, ours stand out for their authenticity and efficacy, containing over 700 active Growth Factors in every exosome, and 100 Billion exosomes per bottle. 

Other products utilize Lyophilized exosomes, an inferior preservation method resulting in a minimal amount of factors. Other products feature Conditioned Media, which is not exosomes. Conditioned Media is just food for stem cells.  Despite a similar price point, our exosomes surpass these products in quality and efficacy, providing far better results with fewer treatments. 

Additionally, our exosomes are the highest quality human UC-MSC exosomes available today, ensuring safety and efficacy. They do not contain DNA, minimizing the risk of adverse immune responses. Our commitment to safety and potency is exemplified by our rigorous adherence to FDA and Government of Canada standards, surpassing industry benchmarks. Making our product not only the gold standard in exosomes but also the ONLY product legally permitted to be administered via injected, added to IV’s and nebulization.

The Benefits of Exosomes from ADVANCED-BIOLOGICS:

For anything an Exosome can do, Advanced-Biologics provides the highest quality, most effective product. At Advanced-Biologics, we take pride in offering the safest and most potent exosome products on the market. Our exosomes harness the remarkable potential of regenerative medicine to promote healing and rejuvenation. They can be used for a wide range of applications, from scarless wound restoration to hair growth, wound care, joint and ligament repair, Botox alternatives, neurodegenerative concerns, organs and a long list of other applications (LINK TO APPLICATIONS). Exosomes from the most potent source, using the most meticulous methods and patents, and delivering over 700 Active Growth Factors per exosome, and over 100 Billion per bottle. There is no other exosome on the market that comes close.

With every batch accompanied by rigorous third-party testing, including Endotoxin and Mycoplasma Reports, Sterility Reports, Nanoparticle Tracking Analysis (N.T.A.), and Proteomics Assays, we guarantee the highest level of regulatory compliance and product quality.

Discover the difference our exosomes can make in your practice or clinic. Try our product today and experience the unparalleled benefits of Advanced-Biologics exosomes. Our exosomes are already being used in 37 countries, including Canada, and by hundreds of doctors and professionals in the US who have treated thousands of patients. Consultations and peer-to-peer discussions with our experienced providers are available to help you unlock the full potential of this transformative technology.

EXOSOME PURIFICATION & ISOLATION METHODS

The key exosome purification and isolation methods summarized:

Ultracentrifugation (UC):

Considered the "gold standard" for exosome isolation

Involves multiple rounds of high-speed centrifugation (100,000 x g) to pellet exosomes

Advantages: Widely used, well-established method

Disadvantages: Time-consuming, requires specialized equipment, potential contamination with non-exosomal particles

Ultrafiltration:

Separates exosomes based on size using membrane filters

Advantages: Simple, no specialized equipment required, handles large sample volumes

Disadvantages: Potential clogging of filters, low recovery rates

Size Exclusion Chromatography (SEC):

Separates exosomes based on size using porous beads in a column

Advantages: Efficient separation without altering exosome properties

Disadvantages: Time-consuming, requires specialized equipment

Precipitation Methods:

Use polymers like polyethylene glycol (PEG) to precipitate exosomes

Advantages: Simple, fast, handles large sample volumes

Disadvantages: Potential co-precipitation of non-exosomal particles

Immunoaffinity Capture:

Uses antibodies to specifically bind and isolate exosomes

Advantages: High specificity, can isolate exosomes from specific cell types

Disadvantages: Expensive, requires specialized equipment and reagents

Commercial Kits:

Various commercial kits available (e.g., ExoQuick, Total Exosome Isolation Reagent)

Advantages: Convenient, time-saving, no specialized equipment required

Disadvantages: Potential co-isolation of non-exosomal particles, expensive for large sample volumes

Emerging Methods:

Microfluidic devices, acoustic/electric manipulation, and other novel techniques

Advantages: High purity, recovery rates, and throughput

Disadvantages: Complex, expensive, not widely available

These methods highlight the importance of efficient, standardized isolation methods that balance purity, recovery, throughput, and cost-effectiveness for exosome research and therapeutic applications.

Embryonic Stem Cells VS. Mesenchymal Stem Cells (MSCs) VS. ADVANCED BIOLOGICS UC-MSC Exosomes

In summary, embryonic stem cells are pluripotent, and have higher proliferative capacity, but raise ethical concerns, while MSCs are multipotent adult stem cells with more limited proliferation. MSC-derived exosomes offer a cell-free, safest approach to harness the maximum regenerative potential of MSCs.

Embryonic Stem Cells vs. Mesenchymal Stem Cells (MSCs):

Potency:

Embryonic stem cells are pluripotent, meaning they can differentiate into cell types of all three germ layers (ectoderm, mesoderm, endoderm)

MSCs are multipotent, meaning they can differentiate into a more limited range of cell types, primarily of the mesodermal lineage such as osteoblasts, chondrocytes, and adipocytes.

Origin and Location:

Embryonic stem cells are derived from the inner cell mass of the blastocyst, an early-stage pre-implantation embryo.MSCs are adult stem cells that can be isolated from various adult tissues, such as bone marrow, adipose tissue, umbilical cord, and others.

Proliferation and Expansion:

Embryonic stem cells have a higher capacity for self-renewal and proliferation compared to adult MSCs.

MSCs have more limited self-renewal and proliferative capacity compared to embryonic stem cells.

Mesenchymal Stem Cells (MSCs) vs. Umbilical Cord MSC-Derived Exosomes ( ADVANCED BIOLOGICS UC-MSC Exosomes):

In summary, while both MSCs and UC-MSC exosomes have regenerative properties, the exosomes offer advantages in terms of ethics, safety, scalability, and superior therapeutic efficacy, making them a more promising cell-free approach for regenerative medicine applications.

Composition:

MSCs are the actual stem cells that can differentiate into various cell types.

UC-MSC exosomes are extracellular vesicles secreted by umbilical cord-derived MSCs, containing a variety of bioactive molecules like proteins, RNAs, and lipids4.

Therapeutic Mechanism:

MSCs can directly differentiate into target cell types to promote tissue regeneration.

UC-MSC exosomes can indirectly stimulate tissue repair and regeneration by transferring their bioactive cargo to target cells, without the need for direct differentiation.

Safety Profile:

MSCs, being living cells, carry a potential risk of uncontrolled proliferation and tumour formation.UC-MSC exosomes, lacking genetic material, do not pose the same safety or ethical concerns as stem cell therapies.

Potency and Regenerative Capacity:

Studies have shown that UC-MSC exosomes exhibit superior regenerative potential compared to MSCs themselves.

UC-MSC exosomes can more effectively restore organ function and promote tissue repair in various disease models.

Scalability and Preservation:

UC-MSC exosomes are easier to scale up production and preserve compared to the challenges associated with MSCs.

RESEARCHES

Your Paragraph text goes Lorem ipsum dolor sit amet, consectetur adipisicing elit. Autem dolore, alias, numquam enim ab voluptate id quam harum ducimus cupiditate similique quisquam et deserunt, recusandae. here

APPLICATIONS:

Anti-Aging: Exosomes are being investigated for their anti-ageing effects on skin and other tissues. They can promote collagen production, reduce oxidative stress, and enhance cellular repair mechanisms, leading to improved skin texture, elasticity, and overall youthfulness.

Hair Restoration: Exosomes have been studied for their potential in promoting hair growth and treating conditions like alopecia. They can stimulate hair follicle proliferation, prolong the growth cycle, and improve hair density and thickness.

Erectile Dysfunction: Exosomes have shown potential in the treatment of erectile dysfunction by promoting penile tissue regeneration, improving blood flow, and enhancing erectile function.

Vaginal Restoration / Rejuvenation: Exosome therapy can offer several potential benefits for vaginal restoration and rejuvenation including Improved Vaginal Tissue Structure and Elasticity, Regulation of Vaginal pH and Prevention of Infections, Promotion of Blood Vessel Growth (Angiogenesis), Anti-Inflammatory Effects, Potential Regulation of Hormone Levels, and of course enhanced sexual comfort, pleasure and sensitivity.

Wound Healing: Exosomes play a crucial role in promoting tissue regeneration and wound healing. They can be used to treat various types of wounds, including acute injuries and chronic ulcers, by stimulating cell proliferation, migration, and angiogenesis.

Orthopaedic Conditions: Exosomes have shown promising results in the treatment of orthopaedic conditions such as osteoarthritis, tendon injuries, and cartilage defects. They can help repair damaged tissues, reduce inflammation, and improve joint function.

Neurodegenerative Diseases: Exosomes have been studied as potential therapeutics for neurodegenerative diseases like Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis (ALS). They can deliver neuroprotective factors and promote neuronal survival and regeneration.

Cardiovascular Disorders: Exosomes hold potential in the treatment of cardiovascular disorders such as myocardial infarction and heart failure. They can enhance cardiac repair, improve cardiac function, and stimulate angiogenesis.

Regenerative Dentistry: Exosomes have emerged as promising agents in regenerative dentistry for applications such as dental pulp regeneration, periodontal tissue repair, and bone regeneration. They can promote odontogenic differentiation, enhance tissue regeneration, and facilitate dental implant osseointegration.Dentists using UC-MSC Exosomes have reported up to 500% increase in healing and recovery time when applied after procedures such as root canals, tooth extractions, and implant procedures.

Regenerative Medicine: Exosomes are being investigated for their regenerative properties in various medical fields, including tissue engineering, organ transplantation, and stem cell therapy. They can facilitate tissue regeneration, modulate the immune response, and promote tissue homeostasis.

Cancer Therapy: Exosomes have emerged as potential therapeutic agents in cancer treatment. They can be used to deliver anti-cancer drugs, suppress tumour growth, and modulate the tumour microenvironment. Exosome-based immunotherapies are also being explored for cancer immunotherapy.

Immune Modulation: Exosomes have immunomodulatory properties that make them promising candidates for the treatment of autoimmune diseases, inflammatory disorders, and transplant rejection. They can regulate immune cell function, suppress inflammation, and promote immune tolerance.

Drug Delivery: Exosomes are being explored as novel drug-delivery vehicles for various therapeutic agents, including small molecules, proteins, and nucleic acids. Their natural targeting ability, biocompatibility, and stability make them attractive candidates for targeted drug delivery systems.

Organ Regeneration: Exosomes hold promise for organ regeneration and repair by promoting stem cell differentiation, tissue remodelling, and angiogenesis. They can be used to regenerate damaged organs, such as the liver, kidney, and heart, and improve overall organ function.

General Health and Maintenance: By harnessing the regenerative, anti-inflammatory, and neuroprotective properties of UC-MSC exosomes, individuals can potentially maintain and build a healthier body by promoting tissue repair, modulating immune responses, and supporting overall cellular function and rejuvenation

These applications demonstrate some of the versatility and therapeutic potential of exosomes across various medical specialities. Ongoing research continues to uncover new insights into their mechanisms of action and expand their clinical applications.

FAQ'S

More information on the potential side effects of using UC-MSC exosomes from the human umbilical cord for regenerative medicine:

In summary, the available evidence indicates that UC-MSC exosomes have a good safety profile, lack tumorigenicity, and exhibit high biocompatibility, making them a promising regenerative medicine approach with minimal side effects. Continued clinical research will further elucidate the long-term safety of these exosome-based therapies.

There does not appear to be significant evidence of major side effects associated with the use of mesenchymal stem cell (MSC) exosomes derived from the human umbilical cord for regenerative medicine applications. The key points are:

Safety of UC-MSC Exosomes:The study in4 specifically evaluated the safety of exosomes derived from human umbilical cord MSCs (hUC-MSCs). It found that hUC-MSC exosomes had a protective effect and did not cause any side effects on liver or renal function in the tested models.

Lack of Tumorigenicity:Unlike stem cell therapies, the search results indicate that UC-MSC exosomes do not contain any genetic material, eliminating the risk of uncontrolled cell proliferation or tumour formation. This is a key advantage of exosome-based regenerative approaches.

Immunogenicity and Biocompatibility: Umbilical cord-derived MSCs, and by extension their exosomes, are known to have low immunogenicity. This makes UC-MSC exosomes a biocompatible option for regenerative medicine without triggering adverse immune responses.

Extensive Preclinical and Clinical Evidence: Research highlights numerous preclinical studies demonstrating the therapeutic potential of UC-MSC exosomes in a wide range of disease models, from wound healing to ovarian regeneration. While more clinical data is still emerging, the existing preclinical evidence suggests a favourable safety profile for these exosome-based therapies.

More about the role of embryonic stem cells in human development

In summary, embryonic stem cells are the foundational building blocks that give rise to the specialized cells and tissues of the human body through a tightly regulated process of differentiation and morphogenesis, which is heavily influenced by the signalling functions of exosomes.

Explanation of the role of embryonic stem cells in human development: Embryonic stem cells (ESCs) are derived from the inner cell mass of the human blastocyst, which is a pre-implantation stage of the embryo1. These ESCs are characterized by their pluripotency - the ability to give rise to cells of all three germ layers (ectoderm, mesoderm, and endoderm). As the human embryo develops, the pluripotent ESCs undergo a process called gastrulation, where they differentiate into the specialized cell types that will form the various tissues and organs. This differentiation is guided by signalling molecules and transcription factors that activate specific developmental pathways.

Importantly, exosomes secreted by the ESCs and differentiating cells are crucial in mediating these signalling pathways and coordinating the complex morphogenesis during embryonic development. The exosomes transfer important bioactive molecules that facilitate intercellular communication and regulate the patterning and segmentation of the embryo.

Through this remarkable self-organization, driven in part by the ESCs and their exosomes, a single fertilized egg can develop into the intricate, multi-organ human body13. The pluripotency of ESCs and their ability to differentiate into all cell types is what enables the formation of the various tissues and organs during embryogenesis.

What are the characteristics of embryonic stem cells?

In summary, the key defining characteristics of embryonic stem cells are their pluripotency, self-renewal capacity, genetic stability, and significant therapeutic potential for regenerative applications.The key characteristics of embryonic stem cells (ESCs) are:

Pluripotency:

ESCs are derived from the inner cell mass of the blastocyst, an early-stage pre-implantation embryo3.ESCs are pluripotent, meaning they can differentiate into cell types of all three germ layers - ectoderm, mesoderm, and endoderm1234.This pluripotency distinguishes ESCs from adult stem cells, which are multipotent and can only produce a limited number of cell types1.

Self-Renewal:

Under defined culture conditions, ESCs are capable of self-renewing indefinitely in an undifferentiated state23.ESCs exhibit remarkable long-term proliferative potential and maintain high telomerase activity2.

Genetic Stability:

ESCs typically have a normal karyotype, unlike embryonal carcinoma (EC) cells which can harbor genetic mutations and abnormal karyotypes2.

Therapeutic Potential:

ESCs are being extensively studied for their potential use in regenerative medicine, disease modelling, and cellular/DNA repair therapies124.The ability to differentiate into a wide range of cell types makes ESCs valuable for treating conditions like diabetes, heart disease, and neurodegenerative disorders12.

What is the difference between embryonic stem cells and adult stem cells

In summary, the key differences are that embryonic stem cells are pluripotent, have greater self-renewal and proliferative capacity, and raise more ethical concerns compared to multipotent and more limited adult stem cells.

The key differences between embryonic stem cells (ESCs) and adult stem cells are:

Origin and Potency:

Embryonic stem cells are derived from the inner cell mass of the blastocyst, an early-stage pre-implantation embryo23.ESCs are pluripotent, meaning they can differentiate into cell types of all three germ layers (ectoderm, mesoderm, endoderm).

Adult stem cells are found in various differentiated tissues and organs in the body, such as bone marrow, brain, and skin.

Adult stem cells are multipotent, meaning they can only produce a limited number of cell types, unlike the broad potential of ESCs.

Self-Renewal and Proliferation:

ESCs can self-renew indefinitely and maintain high telomerase activity under defined culture conditions.

Adult stem cells have more limited self-renewal and proliferative capacity compared to ESCs.

Genetic Stability:

ESCs typically have a normal karyotype, unlike embryonal carcinoma cells which can have genetic mutations and abnormalities.

The genetic stability of ESCs makes them more suitable for therapeutic applications.

Ethical Considerations:

Research on ESCs raises ethical concerns due to the destruction of the blastocyst, which some consider to have moral status.

Adult stem cells do not have the same ethical issues as they can be obtained without destroying human embryos.

How are adult stem cells different from embryonic stem cells?

In summary, the key differences are that embryonic stem cells are pluripotent, and have greater self-renewal and proliferative capacity, but raise more ethical concerns, while adult stem cells are multipotent, and have more limited proliferation, but are easier to obtain ethically. MSC Umbilical Cord Stem cell-derived exosomes offer the greatest, ethically sourced exosomes given they are not harvested from an embryo, and being sourced from the umbilical cord, they are not yet subject to depletion and degradation that comes with age.

The key differences between adult stem cells and embryonic stem cells are:

Potency:

Embryonic stem cells are pluripotent, meaning they can differentiate into cell types of all three germ layers (ectoderm, mesoderm, endoderm).

Adult stem cells are multipotent, meaning they can only differentiate into certain cell types related to the tissue they are found in.

Origin and Location:

Embryonic stem cells are derived from the inner cell mass of the blastocyst, an early-stage pre-implantation embryo.

Adult stem cells are found in various differentiated tissues and organs in the body, such as bone marrow, brain, blood, liver, skin, etc.

Self-Renewal and Proliferation:

Embryonic stem cells have a higher capacity for self-renewal and proliferation compared to adult stem cells.

Adult stem cells have more limited self-renewal and proliferative capacity.

Ethical Considerations:

Research on embryonic stem cells raises ethical concerns due to the destruction of the blastocyst.

Adult stem cells do not have the same ethical issues as they can be obtained without destroying human embryos.